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oligo dt primers  (Vazyme Biotech Co)


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    Vazyme Biotech Co oligo dt primers
    Oligo Dt Primers, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 5451 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primers/HiScript+III+1st+Strand+cDNA+Synthesis+Kit+%2BgDNA+wiper/pm42906688-183-4-16
    Average 99 stars, based on 5451 article reviews
    oligo dt primers - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: A conserved Lsm8-exosome module maintains RNA splicing fidelity to control fungal stress adaptation and virulence.
    Article Snippet: .. The full length ORFs of Lsm2/3/4/8 were amplified from PH-1 cDNA and cloned into bait (pGBKT7) and prey (pGADT7) vectors (Clontech) via ClonExpress II One Step Cloning Kit (C112-01, Vazyme, China) with primers in Table S1, respectively. ..

    Article Title: Severity and deservedness determine signalled trustworthiness in third party punishment.
    Article Snippet: Soybean (Glycine max) forms root nodules to house rhizobial bacteria for biological nitrogen fixation.. The development of root nodules is intricately regulated by endogenous and exogenous cues.. The phytohormones brassinosteroids (BRs) have been shown to negatively regulate nodulation in soybean, but the underlying genetic and molecular mechanisms remain largely unknown.

    Article Title: A PCR-independent approach for mtDNA enrichment and next-generation sequencing: comprehensive evaluation and clinical application
    Article Snippet: .. The specific gene regions in the mitochondrial and nuclear genome were amplified and detected with specific primers (Additional file : Table S1) and 2 × Taq Master Mix (Vazyme, China). ..

    Article Title: Antiviral memory B cells exhibit enhanced innate immune response facilitated by epigenetic memory
    Article Snippet: Transposition reaction was performed with TruePrep DNA Library Prep Kit V2 for Illumina kit (Vazyme, TD501) and incubated at 37 °C for 30 min on a thermomixer with shaking at 1,000 rpm. .. Finally, genome DNA was extracted with phenol-chloroform-isoamyl alcohol and amplified by KAPA HiFi HotStart ReadyMix (KAPA Biosystems, KK2611) with barcoded primers (Vazyme, TD202). .. DNA fragments ranging from 200 to 1,000 bp were selected with VAHT DNA clean beads (Vazyme) for deep sequencing.

    Article Title: A conserved Lsm8–exosome module maintains RNA splicing fidelity to control fungal stress adaptation and virulence
    Article Snippet: .. The full length ORFs of Lsm2/3/4/8 were amplified from PH-1 cDNA and cloned into bait (pGBKT7) and prey (pGADT7) vectors (Clontech) via ClonExpress II One Step Cloning Kit (C112-01, Vazyme, China) with primers in Table S1, respectively. ..

    Article Title: A PCR-independent approach for mtDNA enrichment and next-generation sequencing: comprehensive evaluation and clinical application.
    Article Snippet: .. The specific gene regions in the mitochondrial and nuclear genome were amplified and detected with specific primers (Additional file 1: Table S1) and 2 × Taq Master Mix (Vazyme, China). ..

    Clone Assay:

    Article Title: A conserved Lsm8-exosome module maintains RNA splicing fidelity to control fungal stress adaptation and virulence.
    Article Snippet: .. The full length ORFs of Lsm2/3/4/8 were amplified from PH-1 cDNA and cloned into bait (pGBKT7) and prey (pGADT7) vectors (Clontech) via ClonExpress II One Step Cloning Kit (C112-01, Vazyme, China) with primers in Table S1, respectively. ..

    Article Title: A conserved Lsm8–exosome module maintains RNA splicing fidelity to control fungal stress adaptation and virulence
    Article Snippet: .. The full length ORFs of Lsm2/3/4/8 were amplified from PH-1 cDNA and cloned into bait (pGBKT7) and prey (pGADT7) vectors (Clontech) via ClonExpress II One Step Cloning Kit (C112-01, Vazyme, China) with primers in Table S1, respectively. ..

    Cloning:

    Article Title: A conserved Lsm8-exosome module maintains RNA splicing fidelity to control fungal stress adaptation and virulence.
    Article Snippet: .. The full length ORFs of Lsm2/3/4/8 were amplified from PH-1 cDNA and cloned into bait (pGBKT7) and prey (pGADT7) vectors (Clontech) via ClonExpress II One Step Cloning Kit (C112-01, Vazyme, China) with primers in Table S1, respectively. ..

    Article Title: A conserved Lsm8–exosome module maintains RNA splicing fidelity to control fungal stress adaptation and virulence
    Article Snippet: .. The full length ORFs of Lsm2/3/4/8 were amplified from PH-1 cDNA and cloned into bait (pGBKT7) and prey (pGADT7) vectors (Clontech) via ClonExpress II One Step Cloning Kit (C112-01, Vazyme, China) with primers in Table S1, respectively. ..

    Polymerase Chain Reaction:

    Article Title: Endothelial cell-specific TGM2 deficiency promotes progression of temporal lobe epilepsy by disrupting S1P metabolism and intercellular communication
    Article Snippet: .. Genomic DNA extracted from mouse tail tips was used for PCR genotyping with specific primers (PCR conditions: 2× Taq Master Mix (Vazyme P112-03), 5 min at 95 °C, 20 cycles: 30 s at 98 °C, 30 s at 65 °C (decreasing by 0.5 °C per cycle), 72 °C for 45 s, followed by 20 cycles: 98 °C for 30 s, 55 °C for 30 s, 72 °C for 45 s, and a final extension at 72 °C for 5 min). ..

    Genotyping:

    Article Title: Endothelial cell-specific TGM2 deficiency promotes progression of temporal lobe epilepsy by disrupting S1P metabolism and intercellular communication
    Article Snippet: .. Genomic DNA extracted from mouse tail tips was used for PCR genotyping with specific primers (PCR conditions: 2× Taq Master Mix (Vazyme P112-03), 5 min at 95 °C, 20 cycles: 30 s at 98 °C, 30 s at 65 °C (decreasing by 0.5 °C per cycle), 72 °C for 45 s, followed by 20 cycles: 98 °C for 30 s, 55 °C for 30 s, 72 °C for 45 s, and a final extension at 72 °C for 5 min). ..

    Quantitative RT-PCR:

    Article Title: Transcription factor IRF1 modulates HDAC1 and PANoptosis to regulate preeclampsia progression.
    Article Snippet: First-strand cDNA was synthesized with the HiScript II 1st Strand cDNA Synthesis Kit (R211-01, Vazyme). .. Subsequently, qRT-PCR was performed to detect target genes using SYBR qPCR Master Mix (Q311-02, Vazyme) and specific primers (Table 1) on the LightCycler 480 System (Roche). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Transcription factor IRF1 modulates HDAC1 and PANoptosis to regulate preeclampsia progression.
    Article Snippet: First-strand cDNA was synthesized with the HiScript II 1st Strand cDNA Synthesis Kit (R211-01, Vazyme). .. Subsequently, qRT-PCR was performed to detect target genes using SYBR qPCR Master Mix (Q311-02, Vazyme) and specific primers (Table 1) on the LightCycler 480 System (Roche). ..



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    Image Search Results


    miR-151-3p is a key functional cargo in Exe-Exos mediating anti-apoptotic and antioxidant effects. (A – B) Differentially expressed miRNAs between Exe-Exos and Sed-Exos groups (q < 0.05, |log 2 FC| > 1). (C) RT-qPCR validation of 10 differentially expressed miRNAs. (D) Flow cytometry analysis of apoptosis in OGD/R, OGD/R + Exos, and OGD/R + Exos + miR-151-3p groups. (E) Quantification of apoptotic cell percentage (n = 3 independent cell culture experiments). (F) LDH activity (U/L; n = 5 independent cell culture experiments). (G) WB and (H – M) quantification of p-JNK, JNK, C-Caspase9, Caspase9, C-Caspase3, and Caspase3 protein expression (n = 6 independent biological replicates). Exos exosomes, JNK c-Jun N-terminal kinase.

    Journal: Bioactive Materials

    Article Title: Exercise-derived exosomal miR-151-3p: An innovative anti-inflammatory and antioxidant therapeutic for spinal cord injury

    doi: 10.1016/j.bioactmat.2026.06.009

    Figure Lengend Snippet: miR-151-3p is a key functional cargo in Exe-Exos mediating anti-apoptotic and antioxidant effects. (A – B) Differentially expressed miRNAs between Exe-Exos and Sed-Exos groups (q < 0.05, |log 2 FC| > 1). (C) RT-qPCR validation of 10 differentially expressed miRNAs. (D) Flow cytometry analysis of apoptosis in OGD/R, OGD/R + Exos, and OGD/R + Exos + miR-151-3p groups. (E) Quantification of apoptotic cell percentage (n = 3 independent cell culture experiments). (F) LDH activity (U/L; n = 5 independent cell culture experiments). (G) WB and (H – M) quantification of p-JNK, JNK, C-Caspase9, Caspase9, C-Caspase3, and Caspase3 protein expression (n = 6 independent biological replicates). Exos exosomes, JNK c-Jun N-terminal kinase.

    Article Snippet: The miRNA stem-loop primers (Shanghai Jierui Bioengineering) are listed in .

    Techniques: Functional Assay, Quantitative RT-PCR, Biomarker Discovery, Flow Cytometry, Cell Culture, Activity Assay, Expressing